Validation of CAR γδ T Cells

In order to obtain high-quality CAR-γδ T cells for scientific studies, it is required to test the function and activity of CAR engineered γδ T cells. Creative Biolabs has developed a variety of assays including testing viability, migration, and function of CAR engineered γδ T cells. We guarantee to deliver high-quality and high-activity CAR engineered γδ T cells for global clients for scientific and clinical researches.

CAR γδ T Cell Viability Test

We support a variety of assays to determine the ratio of live and dead CAT-γδ T cells.

• Tetrazolium-based Cell Viability Assays

Tetrazolium-based cell viability assays include MTT, MTS, XTT, WST, and CCK-8. These assays are based on the reduced tetrazolium salts in living cells. Tetrazolium salts can be reduced by living cells to form colored formazans. The color change can be measured by absorbance microplate readers.

• Reduction Potential-based Cell Viability Assays

Resazurin (blue) is a popular dye. It can be reduced by viable cells to form colored resorufin (pink). The change of color is measured in absorbance mode by acquiring absorbance at 570 nm.

• ATP-based Cell Viability Assays

ATP as a cell energy source is proportional to the number of living cells. ATP-based cell viability assays are based on the ATP-dependent luciferase reaction.

CAR γδ T Cell Migration Assay

It is of great importance to assess CAR γδ T cell migration during the development of CAR γδ T cell immunotherapy. The migration of CAR γδ T cells to target tumor microenvironment is crucial for tumor clearance. Creative Biolabs designs and provides optimal solutions to test CAR γδ T cell migration.

CAR γδ T Cell Functional Test

• In Vitro Assay
  • Assay of multiplex cytokine production

  We support the detection of multiplex cytokine production such as interferon-gamma (IFNγ), interleukin-2 (IL-2), and other cytokines based on enzyme-linked immunosorbent assay (ELISA), Luminex multiplex assays, and bead-based flow cytometry.

  • Cytotoxicity test

  The cytotoxicity test is designed to evaluate the killing or toxic effect of CAR-γδ T cells on target tumor cells. We use the co-culture of target tumor cells and CAR-γδ T cells to monitor target cell lysis, effector cell frequency, and activity based on flow cytometric technology. We also use radioactive chromium-release to assess the cytotoxicity of CAR-γδ T cells on target tumor cells.

• In Vivo Assay
  • Construction of Xenograft animal model

  We support the construction of a variety of animal models to study the function of CAR-γδ T cells.

  • Functional evaluation of CAR-γδ T cells on animal models

  We provide comprehensive assays to assess CAR-γδ T cell function on animal models, including efficacy, viability, and bio-distribution testing as well as toxicity evaluation for CAR-γδ T cells.

Fig.1 Effects of NKG2D CAR-expressing Vγ9Vδ2 T cells in a human CRC Xenograft model. (Ang, 2020)

Advantages

• Comprehensive in vivo and in vitro assays for evaluation of the function of CAR-γδ T cells
• Excellent experts providing the best solutions according to your project
• Streamline management to short project cycle
• Best after-sales service to solve all kinds of worries

Please contact us for more details.

Reference

1. Ang, W.X. et al. Electroporation of NKG2D RNA CAR Improves Vγ9Vδ2 T Cell Responses against Human Solid Tumor Xenografts. Molecular Therapy: Oncolytics. 2020, 17:421-430.