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As a pioneer and the undisrupted global leader in antibody discovery and engineering, Creative Biolabs proudly introduces our professional service of anti-membrane protein antibody discovery with our diverse technologies.

Membrane proteins, which consist of 20~30% of total encoding genes in the genome, play a critical role in multiple biological processes, including ligand-receptor binding, signal transduction, molecule transportation, intercellular recognition, and cell adhesion. Currently, there rises growing interest regarding membrane proteins as promising targets for various drug development and other applications.

To obtain highly functional anti-membrane protein antibodies, expression and presentation of the membrane protein of interest as immunogen is the prerequisite. As most membrane proteins are partially or completely embedded in the lipid bilayer, traditional expression methods meet obstacles in presenting the native conformations, such as low expression level, difficult extraction, misfolding, and incomplete post-translational modifications. With a full understanding of the critical requirements in this field, Creative Biolabs has developed a novel Magic? platform, which enables high-efficient expression of membrane proteins and following antibody development routes based on our multiple impeccable technologies.

To obtain highly functional anti-membrane protein antibodies, expression and presentation of the membrane protein of interest as immunogen is the prerequisite. As most membrane proteins are partially or completely embedded in the lipid bilayer, traditional expression methods meet obstacles in presenting the native conformations, such as low expression level, difficult extraction, misfolding, and incomplete post-translational modifications. With a full understanding of the critical requirements in this field, Creative Biolabs has developed a novel Magic? platform, which enables high-efficient expression of membrane proteins and following antibody development routes based on our multiple impeccable technologies.

Cell-based Membrane Protein Expression

Creative Biolabs has developed a cell-based membrane protein expression system, which can yield membrane proteins of interest from a recombinant plasmid in living cell system. This platform is highly flexible for multiple options of host organisms, including:

• Membrane Protein Expression in E. coli
• Membrane Protein Expression in Yeast Cells
• Membrane Protein Expression in Baculovirus-Insect Cells
• Membrane Protein Expression in Mammalian Cells

Anti-Membrane Protein Antibody Production Using VLPs

We can genetically integrate the indicated membrane protein (especially viral coat proteins) on virus-like particles (VLP), a kind of self-assembling but non-infectious virus.

Membrane Proteins Reconstituted in Micelles

Detergent-solubilization is a routine procedure for membrane protein isolation. Selection of appropriate detergent is very critical as misuse of detergent might compromise protein structure, resulting in denatured products. Based on our specific reagents together with other commercially available detergents, we can perform up to 20 conditions to screen for the optimal solubilization outcome. As an alternative, we also offer detergent-free plan using our cell-free platform in case detergent use may cause inevitable problems.

Creative Biolabs also provides DNA immunization to discover novel anti-membrane protein antibodies. With preliminary well-characterized protein sequences, this strategy can avoid antigen preparation process and maximize the likelihood of generating and maintaining the native protein structure. It has been proved exceptionally effective in mounting strong antibody response for the challenging targets.

References

1. Sarma, J. V.; Ward, P. A. The complement system. Cell and tissue research. 2011, 343(1): 227-235.
2. Leslie, R. G. Q.; Hansen, S. Complement receptors. 2001.