γδ T Cell Isolation Services

Creative Biolabs is a world-leading service provider that focuses on the research of γδ T cells. With highly skilled and committed experts and state-of-the-art technologies, we are fully confident in offering the best γδ T cell isolation services to support the research of γδ T cell biology and the development of γδ T cell-based immunotherapies.

γδ T cells have been regarded as promising candidates for the development of γδ T cell-based therapy for cancer treatment due to their unique anti-tumor features such as their potent cytotoxicity against tumors and production of cytokines. It is a very important step for studying the biological function of γδ T cells and developing γδ T cell-based therapy to isolate and purify functional and specific γδ T cell populations from a complicated biological sample.

Technologies for γδ T Cell Isolation

Magnetic bead cell sorting (MACS) is a common, efficient, and fast method to isolate untouched γδ T cells from human peripheral blood mononuclear cells (PBMCs). The basic principle is to deplete non-γ/δ T cells using magnetic particles labeled by antibodies recognizing specific cell surface markers. Non-γ/δ T cells such as α/β T cells, NK cells, B cells, granulocytes, monocytes, dendritic cells, stem cells, and erythroid cells are indirectly labeled with a cocktail of biotin-conjugated monoclonal antibodies. MicroBeads are used to capture these labeled non-γ/δ T cells. Subsequently, the labeled non-γ/δ T cells are retained on a MACS column in the magnetic field of a MACS separator, while the unlabeled γ/δ T cells pass through the column. These untouched γ/δ T cells are very suitable for subsequently phenotypical and functional analysis, such as studying cytokine expression and receptor signaling, antigen recognition, cytotoxicity.

Services

Creative Biolabs is committed to offering comprehensive, one-stop γδ T cell isolation services from fresh or previously frozen PBMCs with high quality and low cost. Service flow is as follows:

Sample preparaton Magnetic labeling Magnetic separation Evaluation of γ/δ T cell purity

Prepare a PBMC suspension from whole peripheral blood by density gradient centrifugation

Label non-γδ T cells with a series of biotinconjugated McAbs

MS and LS columns XS columns AUTOMACS separator

Flow cytometry Fluorescence microscopy

Besides, we also offer custom services to support γδ T cell isolation from other samples such as murine and human skin as well as small intestinal intraepithelial lymphocytes. We guarantee to deliver high-quality, high-pure, and untouched γδ T cell products for downstream analysis and research.

Advantages

• An excellent expert team assigned to your project to support all-around services
• One-stop solution: streamlined strategy from sample preparation to top-quality γδ T cell product delivery.
• Rapid order processing and fast turn-around times
• Isolated γδ T cells suitable for studying γδ T cell biology and developing γδ T cell-based immunotherapies

If you are interested in our γδ T cell isolation services, please contact us for more details.