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With in-person events back again, Creative Biolabs is excited to greet you at the upcoming international conferences. Meet our team at 2022 BIO International Convention in June (booth #1754) and at the 13th Annual World ADC San Diego in September (booth number to be updated) for expert consultation on your drug discovery. Shoot an email to arrange an in-person meeting!

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γδ T Cell Service

γδ T Cell Cytotoxicity Test

γδ T Cell Cytotoxicity Test

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In recent years, Gamma delta (γδ) T cells have become a potential avenue for adoptive immunotherapy. To date, a number of clinical trials have used γδ T cells for cancer treatment. And this type of cell has special characteristics: MHC-unrestricted cytotoxicity, secrete inflammatory cytokines, ability to distinguish transformed cells from normal cells, as well as promote the formation of antigen-specific CD81 and CD41 T cell response. Due to the important function of γδ T cell in several diseases, it is necessary to develop suitable clinical assays for γδ T cell activity and cytotoxicity.

Creative Biolabs is a leader in the research of γδ T cells and cancer immunotherapy, we provide comprehensive γδ T cells cytotoxicity test services, including LDH cytotoxicity test, flow cytometry-based cytotoxicity test, chromium-51 release assay, impedance-based label-free real-time cytotoxicity assay…

LDH Cytotoxicity Test

γδ T Cell Cytotoxicity Test

The LDH-based cytotoxicity assay is one of the most commonly used methods for cell cytotoxicity detection. Lactate dehydrogenase (LDH) is a soluble cytosolic enzyme that exists in most eukaryotic cells, and when the plasma membrane is damaged upon cell death, LDH releases into the culture medium. It is a well-established and reliable indicator of cellular toxicity and can be measured by a coupled enzymatic reaction in which LDH catalyzes the conversion of lactate to pyruvate via NAD+ reduction to NADH. Then the diaphorase uses NADH to reduce a tetrazolium salt (INT) to a red formazan product that can be detected at 490 nm.

Flow Cytometry-Based Cytotoxicity Test

As an alternative to the standard chrominum-51 (51Cr) release assays, flow cytometry-based cytotoxicity test also can be used for the γδ T Cell Cytotoxicity detection. To perform the flow cytometry-based cytotoxicity assay, the γδ T Cells should be labeled with a cell tracking dye- carboxyfluorescein diacetate succinimidyl ester (CFSE) and incubated with effector cells. Then, 7-AAD, which binds to DNA and can only enter membrane-compromised cells, is used as an indicator of cell death.

Flow cytometric analysis of dead cell stain. Fig.1 Flow cytometric analysis of dead cell stain. (Kandarian, 2017)

Impedance-Based Label-Free Real-Time Cytotoxicity Assay

The impedance-based label-free real-time cytotoxicity assay is developed based on electrical impedance measurements and enables to achieve the real-time monitoring of T-cell-mediated cytotoxicity. This method has several advantages, such as easily feasible, sensitive and reproducible; compared to classical chromium release assay, no radioactivity is required and extended incubation periods are possible…

γδ T cells are being exploited increasingly for cancer immunotherapy. Based on our advanced technology and abundant experience, Creative Biolabs offers high-quality γδ T cells cytotoxicity test services. We also provide other customized services covering the entire γδ T cell development process to best suit your technical, program, and budget requirements. Please contact us for your exclusive solution.

Reference

  1. Kandarian, F., et al. A flow cytometry-based cytotoxicity assay for the assessment of human NK cell activity. JoVE (Journal of Visualized Experiments). 2017, (126), e56191.
All listed services and products are for research use only. Do not use in any diagnostic or therapeutic applications.

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