Flow Cytometry-Based Cytotoxicity Test

Creative Biolabs is a pioneer in the γδ T cell therapy field. With several years of exploration, we have built a comprehensive and advanced γδ T cell cytotoxicity test platform. We provide different strategies for γδ T cell cytotoxicity test according to your special needs.

Introduction to γδ T Cell Cytotoxicity

As a unique lymphocyte population and a subset of T cells, human γδ T cells express the γδ T cell receptor (TCR) and they have a specific tissue distribution and antigen (Ag) recognition pathway. To achieve early immunity against infection, γδ T cells take part in many pathways, such as soluble proinflammatory molecules, influenza viruses, chemokines, rapid cytotoxicity or crosstalk with other cell types that are involved. Besides, a previous study has demonstrated that γδ T cells from patients infected with HIV showed antiviral potential via their cytolytic functions. Thus, the most important mechanism of γδ T cell-mediated immune responses to infection is the direct killing of infected cells or invasive pathogens.

Many pathways are involved in the cytotoxicity of γδ T cells, such as Fas‑Fas ligand interactions, and the secretion of granzyme B, perforin, and granzyme M. Moreover, in several diseases, defects in these cytotoxic mechanisms lead to the suppression of γδ T cell activity. But researches have shown that stimulation with phosphoantigen promotes the function of γδ T cells and may inhibit HIV infection via cell‑release antiviral factors.

Flow Cytometry-Based Cytotoxicity Test

Due to the crucial role of γδ T cell in infectious disease, it is necessary to find a suitable clinical assay for γδ T cell activity determination. The commonly used cytotoxicity assay is Chromium-51 release assay, however, this method is low sensitivity, time-consuming, and needs to use hazardous radiation. Flow cytometry-based cytotoxicity test is a novel method that has been developed in recent years. This method can accelerate the analysis to be conducted at the single-cell level, and compared to the method needed to release a preloaded marker, it may be more convenient for routine application. In Creative Biolabs, the flow cytometry‑based assays use forward and side scatter characteristics for the discrimination of different cell populations and distinction between dead and viable cells, or utilize differential labeling of target cells with a range of fluorescent dyes.

Fig.1 Flow cytometric analysis of γδ T cell cytotoxicity assay. (Jin, 2018)

Services at Creative Biolabs

With years of experience and advanced technologies, Creative Biolabs is confident in providing the best γδ T cell cytotoxicity test services for our clients, including flow cytometry-based cytotoxicity test. Our experienced scientists are dedicated to promoting your project to the clinical stage.

Please contact us for more information.

Reference

1. Jin, Q., et al. Rapid flow cytometry-based assay for the evaluation of γδ T cell-mediated cytotoxicity. Molecular medicine reports. 2018, 17(3), 3555-3562.