LDH Cytotoxicity Test

In the in vitro studies of biological evaluation, cytotoxicity is one of the most important indicators. Drugs and pesticides usually have different cytotoxicity mechanisms in vitro, for example, prevention of protein synthesis, destruction of cell membranes, as well as irreversible binding to receptors. As one of the most experienced and responsible companies in the T cell therapy development field, Creative Biolabs provides a range of γδ T cell cytotoxicity test services for our clients, including lactate dehydrogenase (LDH) cytotoxicity test.

Introduction to Lactate Dehydrogenase (LDH) Assay

LDH, a ubiquitously-expressed intracellular enzyme, catalyzes the reversible oxidation of lactate to pyruvate. LDH can release into the supernatant when cell damage or lysis, because this enzyme mostly exists in the cytosol. Hence, LDH is usually used to measure cytotoxicity and cell number in the in vitro cell culture systems. Nowadays, detecting levels of LDH enzyme has become a routine and fundamental method of study the cytotoxicity effects of experimental drug formulations and cell transfection. In Creative Biolabs, our LDH cytotoxicity test enables accurate LDH levels determination in cultured cell supernatants from a wide range of cell lines by using a simple, proven colorimetric (UV) enzymatic assay to specifically detect LDH.

Fig.1 Comparison of % cell viability as an indicator of lactate dehydrogenase release activity of BioPure MTAD compared with 1%, 3%, and 5% sodium hypochlorite at 1, 5, 10, and 15 min time intervals. (Singh, 2018)

Services at Creative Biolabs

Several previous studies have detected γδ T Cell cytotoxicity through the LDH Cytotoxicity assay. For example, in an analysis of tumor-infiltrating γδ T cells in rectal cancer, the LDH assay was done. In order to detect specific cytotoxicity, HR8348 and Vδ2 T cells were co-cultured at the ratios of 10:1, 20:1 and 30:1. Results showed that the inhibitory effects of Vδ2 T cells from para-carcinoma tissues and from rectal cancer tissues were not significantly different.

With years of exploration and development in the immunotherapy field, Creative Biolabs has built a comprehensive γδ T cell cytotoxicity test platform. Several cell cytotoxicity tests are available in our lab, such as LDH Cytotoxicity Test, Flow Cytometry-Based Cytotoxicity Test, Chromium-51 Release Assay, Impedance-Based Label-Free Real-Time Cytotoxicity Assay. We have professional project management, and advanced technologies, we can provide you with a comprehensive solution package to promote your projects success.

Advantages

• Extensive experience in the analysis of γδ T Cell Cytotoxicity
• Well-established γδ T Cell Cytotoxicity Test platform
• Reliable data delivery and interpretation
• Excellent experts provide real-time technical guidance

Creative Biolabs has a team of experienced scientists to provide the best strategy and protocols for γδ T cell migration assay services. Please contact us for more information.

Reference

1. Singh, A., et al. Comparative evaluation of cytotoxic effects of MTAD and sodium hypochlorite using lactate dehydrogenase and trypan blue assays: An in vitro study. Saudi Endodontic Journal. 2018, 8(3), 189.