γδ T Cell Migration Assay

As one of the most important procedures in several biological processes, cell migration should be monitored. Usually, cell migration can be seen in tissue formation, embryological development, cancer progression, as well as inflammation. Creative Biolabs is a global company that focuses on T cell therapy discovery and development. With our extensive experience and unique platform, we can provide γδ T cell migration assay for customers all over the world. Our customized services will save your time and budget, and will promote your project to a big success.

Introduction to Cell Migration

Cell migration is a crucial process in the development and maintenance of multicellular organisms. Methods to study cell migratory behavior are very useful research tools for many disciplines in biomedical sciences, bioengineering, biology, and related fields. Besides, the study of cell migration is also very important in cancer research, because the main cause of death in cancer patients contacts with metastatic progression. Typically, cancer cells must migrate and invade through the extracellular matrix (ECM), then they can spread and disseminate throughout the body, and finally extravasate to form distant foci. Until now, several methods have been developed to study cell migration, such as cell culture wound-closure and transwell migration and invasion assays.

Cell Migration Assay for γδ T Cell

Migration of γδ T cells is related to the combined actions of various adhesion molecules, and also regulated by the interactions between chemokines and their receptors. Previous studies demonstrated that circulating γδ T cells migrate in response to chemokine regulated upon activation normal T-cell expressed and secreted (RANTES), macrophage inflammatory protein 1α (MIP-1α), MIP-1β, and IP-10. With years of experience in γδ T Cell research, Creative Biolabs provides high-quality γδ T cell migration assays:

• Boyden Chamber assay

Boyden Chamber assay is the most widely used cell migration assay. This assay makes use of a hollow plastic chamber, sealed at one end with a porous membrane. The chamber should suspend over a larger well which may have medium and/or chemoattractants. Cells are cultured inside the chamber and enabled to migrate through the pores, to the other side of the membrane. Then, migratory cells can be stained and counted.

Fig. 1 Illustration of a migration assay using a Boyden chamber. (Lautenschlaeger, 2011)

• Cell Culture Wound Closure Assay

Cell culture wound closure assay is a commonly used strategy for in vitro cell migration detection. In this assay, a cell-free area should be produced in a confluent monolayer via physical exclusion or through removing the cells from the area by chemical, mechanical, or thermal damage. The exposure to the cell-free area leads the cells to migrate into the gap which can be observed by a transmitted-light microscope equipped.

Fig.2 Silicone insert for gap creation via cell exclusion in a standard 24-well plate. (Jonkman, 2014)

Creative Biolabs has a team of experienced scientists to provide the best strategy and protocols for γδ T cell migration assay services. Please contact us for more information.

References

1. Jonkman, J. E., et al. An introduction to the wound healing assay using live-cell microscopy. Cell adhesion & migration. 2014, 8(5), 440-451.
2. Lautenschlaeger, F. Cell compliance: cytoskeletal origin and importance for cellular function. 2011. (Doctoral thesis). https://doi.org/10.17863/CAM.16575