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γδ TCR Service

γδ TCR Generation Services

γδ TCR Generation Services

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γδ T Cell -- a Unique Cell Relative to αβ T Cell

Cellular immunity mediated by T lymphocytes plays a central role in both initiate and adaptive immune responses. There are multiple subgroups of T lymphocytes, which are categorized into αβ T cells and γδ T cells distinguished by the expression of the featured αβ or γδ T cell receptor (TCR) on the cell surface. The common αβ T cells and special γδ T cells originate from a common progenitor in the thymus but are fundamentally different in surface markers, distributions, antigen recognition, and functions, both of which coordinate and cooperate to induce a diversity of immune responses against pathogens and antigens.

Table.1 Comparison of αβ and γδ T cells.

αβ T cells γδ T cells
TCR αβ-TCR with α chain and βchain γδ-TCR with γ chain n and δ chain
Proportion ~95% <5%
Distribution Peripheral blood, lymph nodes, and tissues Mucosa or epithelial tissue
Antigen recognition Antigen processing and (MHC) presentation In an MHC unrestricted manner
Antigens Non-self peptides Phosphoantigens, MHC, and CD1 related molecules, soluble proteins, etc.
Co-receptors CD4 and CD8 lineage markers Natural killer receptors and other receptors
TCR diversity Relatively vast Limited
Functions Various cellular immune response Mainly involved in the inflammatory response and tumor immune surveillance

TCR Generation for γδ T Cell Therapy Development

Be it αβ T cells or γδ T cells, the activation and various functions of T lymphocytes are mediated by the surface TCR, a heterodimer with two subchains expressed as part of a complex with the CD3. The genetic recombination yields extensive diversity in TCRs, which bind to antigens and induce effector functions in the way similar to antibodies. In recent years, generation, modification, and cloning of TCR with high specificity and affinity by recombinant technology have shown great value in clinical and therapeutic applications, such as TCR-engineered T cell therapy and TCR gene transfer therapy.

For efficient engineered γδ T cell and γδ T cell therapy development, it is necessary to first obtain TCR (both αβ-TCR and γδ-TCR) or TCR-gene modified γδ T cells. γδ TCR generation usually begins with selecting an appropriate antigen to stimulate and activate γδ T cells. Then TCR sequence identification and cloning are conducted to obtain enough specific TCR genes. The constructed the TCR vector plasmids or lentivirus or retrovirus vectors will then be transfected into tool cells to express antigen-specific TCRs, or transfected into γδ T cells to develop TCR-engineered γδ T cells.

Rapid cloning and expression of human TCRαβ or TCRγδ in a retroviral vector. Fig.1 Rapid cloning and expression of human TCRαβ or TCRγδ in a retroviral vector. (Guo, 2016)

Our Featured γδ TCR Generation Services

As an expert in γδ T cell therapy development, Creative Biolabs has established an advanced technology platform aiming to provide comprehensive custom TCR generation services, including both αβ-TCR and γδ-TCR, for engineered γδT cell or γδ T cell immunotherapy development. Clients just offer us the specific antigen information or basic gene information of interest TCR, our one-stop TCR generation services can start from the design and synthesis of the target TCR gene.

If you are interested in or have any questions, just directly contact us and our seasoned scientists will offer you tailored solutions and support to pull you out of troubles.

Reference

  1. Guo, X.Z., et al. Rapid cloning, expression, and functional characterization of paired αβ and γδ T-cell receptor chains from single-cell analysis. Molecular Therapy-Methods & Clinical Development. 2016, 3: 15054.
All listed services and products are for research use only. Do not use in any diagnostic or therapeutic applications.

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