γδ TCR Repertoires Analysis Services

Creative Biolabs concentrates on promoting the understanding of gamma delta (γδ) T cell receptors breakthroughs in disease therapy. We will combine γδ T cell receptor (TCR) repertoires analysis research with other incredible projects around the world to boost novel γδ T cell development in unprecedented ways. In our company, our scientists specialized in γδ T cell studies will work with you to develop the most appropriate strategy that will offer the reproducible data for your research.

Introduction to γδ TCR Repertoires

γδ T cells are a rare type of T cells that are of great importance in cytokine production, tissue regulation, and immune defense against tumors. γδ T cells express specific γδ TCR that consists of a γ-chain and a δ-chain. The genes encoding these two chains of γδ TCR are rearranged in the thymus. During this process, the variable (V) domain, the diversity (D) domain, and the joining (J) domains of somatic DNA recombine and create the diversity of γδ TCR chains. As a result, all γδ T cells are merged to form large γδ TCR repertoires. Moreover, pilot studies have indicated that the composition of specific γδ TCR repertoires is associated with the disease development in the human body. However, only a few TCR ligands, proteins, or factors have been confirmed that can be used for promoting the γδ TCR repertoire and activation.

In this condition, many attempts have been made to generate novel technologies for analyzing human γδ TCR repertoires, identifying the γδ TCR ligands, as well as illustrating the mechanism of TCR diversity in disease models. For example, high-throughput TCR repertoire sequencing and next-generation sequencing (NGS) technology have become powerful tools in much academic research.

Fig.1 Interaction between an antigen-presenting cell (APC) and a T cell, and V(D)J recombination. (Rosati, 2017)

γδ TCR Repertoires Analysis Services

Currently, γδ T cells have been considered as unique innate-like T cells with TCRs that can easily recognition of highly conserved antigens. Meanwhile, evidence has suggested that analyzing the γδ TCR repertoire is important to obtain meaningful data for the immune system features, the causes, and the progression of the disease. At present, the main obstacle to the problem of the γδ TCR repertoire services is that traditional methods are not sufficient to characterize the TCR repertoire in-depth.

To solve this problem, Creative Biolabs has established a panel of assays for the analysis of the γδ TCR repertoire. Currently, we can provide a seamless service chain, ranging from gene expression profiling, γδ TCR repertoire analysis, γδ T cell subsets identification, to development and maintenance of γδ T cell in various disease models. For instance, monoclonal antibodies have been used for detecting specific V gene subgroups of γδ TCR repertoire. A wide variety of polymerase chain reaction (PCR) strategies have been generated for offering a fast and accurate overview of the repertoire.

Besides, next-generation sequencing (NGS) based technologies are most widely employed recently for clonotype identification and γδ TCR repertoire analysis. In our labs, the high-quality RNA libraries from different samples, such as human peripheral blood mononuclear cells (hPBMCs) and purified T cells, will be established to capture the V(D)J variable regions of TCR transcripts and testing the alpha- and beta-chain diversity of γδ TCR.

Fig.2 Exemplary workflow of three principal methodologies for TCR library preparation. (Rosati, 2017)

Features of γδ TCR Repertoires Analysis

• Full-length sequences of complete V(D)J regions
• Flexible sample processing
• Ready-to-use TCR libraries
• Professional knowledge of TCR-gama and TCR-delta
• Excellent sensitivity and reproducibility
• Integrated solution based on multiple methods

Creative Biolabs is recognized as the world leader for providing the most diverse portfolio of γδ TCR repertoires analysis studies for worldwide customers. We are very proud of providing high-quality, omnidirectional γδ T cell therapy development services to remove the difficulties of your projects. For more information, please feel free to contact us or send us an inquiry.

Reference

1. Rosati, E., et al. Overview of methodologies for T-cell receptor repertoire analysis. BMC biotechnology. 2017, 17(1): 61.