CAR Transduction for Engineered γδ T Cell

In the years spent in the development and production of CAR engineered γδ T cells, Creative Biolabs has acquired solid know-how in the field of the development of CAR-γδ T cells. The expertise offered to customers includes custom CAR generation for engineered γδ T cell, γδ T cell preparation, CAR transduction, and validation of CAR γδ T cells. Especially, we have accumulated rich experience in CAR transduction in γδ T cells. We can flexibly utilize a variety of transduction strategies for CAR transduction in γδ T cells.

Electroporation

Electroporation is a physical transfection method that uses high-voltage electric shocks to create temporary pores in cell membranes through which exogenous nucleic acids pass into cells. It is a highly efficient and widely used approach in the field of biological research. Foreign nucleic acids can be introduced into many cell types, including bacteria and mammalian cells, by using the electroporation technique. In the development of CAR engineered γδ T cells, the CAR genes can be introduced into γδ T cells through electroporation technique.

Retroviral Vector

Retroviral vectors provide highly efficient gene delivery vehicles that are widely used in gene therapy. They have been genetically engineered to deliver therapeutic genes to target cells. The most important advantage of retroviral vectors over other vectors is that retroviral vectors can transform their single-stranded RNA genome into double-stranded DNA molecules. Retroviral vectors can be used to permanently modify the nuclear genome of the host cell. With the aid of retroviral vectors, the in vitro constructed CAR genes with less than 8-10 kB length can be introduced into γδ T cells. But a major disadvantage of retroviral vectors is that they only infect dividing cells.

Lentiviral Vector

Lentiviral vectors are also a powerful gene delivery system. It is used to permanently integrate foreign genes into target cells. Due to their ability to infect non-dividing cells, lentiviral vectors have a wider range of potential applications. They can be used to introduce libraries of complementary DNAs, short hairpin RNAs, and cis-regulatory elements into various types of cells including dividing and non-dividing. The in vitro constructed CAR genes can also be integrated into γδ T cells through lentiviral vectors.

Advantages

• Excellent scientists providing a variety of solutions for CAR transduction
• Advanced technical platform to meet the requirement of different projects
• Real-time guidance from start to finish of the project
• One-stop services to remove your worries

If you are interested in our services, please contact us to discuss your project.