CAR γδ T Cell Functional Test

Recent reports have shown that the development of CAR TCR-engineered γδ T cells is a promising method in cellular immunotherapy for the treatment of cancer. However, determining the specific function of engineered γδ T cells also plays an important role in cell development. As a professional CRO company in cell therapy, Creative Biolabs provides customized CAR γδ T cell functional test services to meet our clients’ requirements.

Introduction to CAR Engineered γδ T cell

Rischer et al. demonstrated that peripheral blood-derived Vγ9Vδ2 T cells transduced with retroviral vectors encoding either GD2 or CD19-specific CARs had high CAR expression and could be readily expanded. In vitro tests suggested that CAR-expressing γδ T cells might serve as potent and specific antitumor effector cells. Based on these studies, researches and applications of CAR γδ T cells have been in rapid development. Typically, first-generation CARs had a single CD3-z intracellular signaling domain; they were shown to have poor persistence in vivo. Second-generation and third-generation CARs were created by the addition of one or two costimulatory domains to the CD3-z motif to provide tailored signals to enhance activation and survival. Additionally, because CAR γδ T cells can act as professional antigen-presenting cells, combination therapies with other modalities of immunotherapy such as checkpoint inhibitors, oncolytic viruses, vaccines, or cytokines could synergistically amplify recruitment and function of tumor-infiltrating lymphoid and non-lymphoid cells.

Multiple Functions of γδ T Cell

While γδ T cells clearly show potent antitumor activity, some reports describe the regulatory function of these cells in the tumor microenvironment. Moreover, the application of γδ T cells for CAR T cell cancer therapy has many functional advantages (Figure.1). Thus, the cell functional test is necessary during the process of CAR engineered γδ T cell development. With extensive industrial experience, Creative Biolabs provides high-quality CAR engineered γδ T cell functional test services to global customers.

Fig.1 Functional advantages of γδ T cells for CAR T cell cancer therapy. (Mirzaei, 2016)

Services at Creative Biolabs

The functional test of γδ T cells is a systematical process. Creative Biolabs has a team of experienced Ph.D. level scientists providing professional and attentive customize services. Besides, we have established an advanced platform with various technologies that ensure our customers enjoy the worry-free one-stop services. The technology services our platform offers including but not limited to:

• Flow cytometry
• Animals and in vivo model development
• Cytokine levels and cytotoxic assays
• Statistical analysis

If you need more information, please feel free to contact us.

Reference

1. Mirzaei, H. R.; et al. Prospects for chimeric antigen receptor (CAR) gamma delta T cells: A potential game changer for adoptive t cell cancer immunotherapy. Cancer Lett. 2016, 380(2): 413-23.