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Validation of αβ TCR-γδ T Cells
Online InquiryAfter we successfully used αβ TCR engineering to construct γδ T cells, in order to better understand the impact of γδ T cells on health and disease, the identification and verification of these γδ T cells is also crucial.
Background
The adoptive transfer of genetically engineered T cells has become one of the most promising immunotherapies for many diseases such as cancer. In cancer patients who are resistant to other therapeutic interventions, a large number of trials have shown objective clinical response and even complete remission, after adoptive cell metastasis. The gene relocation of T cells to cancer can be achieved through T cell receptor (TCR) transduction which is specific to the selected antigen.
Vertebrate TCR exists as a heterodimer consisting of αβ or γδ TCR chains. The targets recognized by human γδ T cells tend to be proteins expressed on the cell surface under generalized cell stress (including malignant transformation). A notable exception to this rule is the recognition of pyrophosphate metabolites in the mevalonate pathway by the main peripheral blood subpopulation of γδ T cells expressing TCR composed of Vγ9 and Vδ2 chains. Since there is no evidence that major histocompatibility complex (MHC) restricts γδ T cells, and their targets are expressed in a wide range of diseases, such as cancer and autoimmune diseases, γδ TCRs provide exciting potential for general population immunotherapy.
Fig.1 Unconventional immunity provided by γδ T cells. (Eriksson, 2019)
Validation of αβ TCR-γδ T Cells Services
Comparing the different validation assays of γδ T cells will help to clarify the function of γδ T cells in human disease models (including infection, autoimmunity and cancer). At Creative Biolabs, we provide a series of verification services covering in vivo and in vitro experiments to better meet your needs. These methods include but are not limited to:
Detection of antigen expression changes or atypical patterns. γδ T cells have antigen expression patterns associated with T cells, which distinguish them from the existing αβ T cells. |
Detect the mRNA expression level of TCR γδ T cell representative genes. |
For mRNA-based γδ TCR profiling analysis, paired-end Illumina sequencing was performed. |
In order to evaluate the cytotoxicity of γδ T cells, 51-Chromium release assay was used for verification. |
The use of specific γδ TCR in diseases allows for targeting of the intracellular proteome and/or metabolome. |
Establish specific animal models for different diseases, and the effects of γδ T cells in vivo were verified by in vivo imaging system, immunohistochemistry or other methods. |
Relying on our expertise in extensive analysis, identification and combination technologies, in the validation of αβ TCR-γδ T cells, Creative Biolabs can verify candidate molecules by combining a variety of experimental methods. If you are interested in our services, please feel free to contact us for more detailed information.
Reference
- Eriksson, E. M., Davey, M. S. γδ T cells take the stage. Clinical And Translational Immunology. 2019, 8(11).
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