Validation of αβ TCR-γδ T Cells

Online Inquiry

After we successfully used αβ TCR engineering to construct γδ T cells, in order to better understand the impact of γδ T cells on health and disease, the identification and verification of these γδ T cells is also crucial.


The adoptive transfer of genetically engineered T cells has become one of the most promising immunotherapies for many diseases such as cancer. In cancer patients who are resistant to other therapeutic interventions, a large number of trials have shown objective clinical response and even complete remission, after adoptive cell metastasis. The gene relocation of T cells to cancer can be achieved through T cell receptor (TCR) transduction which is specific to the selected antigen.

Vertebrate TCR exists as a heterodimer consisting of αβ or γδ TCR chains. The targets recognized by human γδ T cells tend to be proteins expressed on the cell surface under generalized cell stress (including malignant transformation). A notable exception to this rule is the recognition of pyrophosphate metabolites in the mevalonate pathway by the main peripheral blood subpopulation of γδ T cells expressing TCR composed of Vγ9 and Vδ2 chains. Since there is no evidence that major histocompatibility complex (MHC) restricts γδ T cells, and their targets are expressed in a wide range of diseases, such as cancer and autoimmune diseases, γδ TCRs provide exciting potential for general population immunotherapy.

Unconventional immunity provided by γδ T cells. Fig.1 Unconventional immunity provided by γδ T cells. (Eriksson, 2019)

Validation of αβ TCR-γδ T Cells Services

Comparing the different validation assays of γδ T cells will help to clarify the function of γδ T cells in human disease models (including infection, autoimmunity and cancer). At Creative Biolabs, we provide a series of verification services covering in vivo and in vitro experiments to better meet your needs. These methods include but are not limited to:

  • Flow Cytometry

Detection of antigen expression changes or atypical patterns. γδ T cells have antigen expression patterns associated with T cells, which distinguish them from the existing αβ T cells.

  • Real-Time Polymerase Chain Reaction (RT-PCR)

Detect the mRNA expression level of TCR γδ T cell representative genes.

  • High-throughput Sequencing

For mRNA-based γδ TCR profiling analysis, paired-end Illumina sequencing was performed.

  • Cytotoxicity Assay

In order to evaluate the cytotoxicity of γδ T cells, 51-Chromium release assay was used for verification.

  • Proteomics and/or Metabolomic Analysis

The use of specific γδ TCR in diseases allows for targeting of the intracellular proteome and/or metabolome.

  • Customized Animal Models

Establish specific animal models for different diseases, and the effects of γδ T cells in vivo were verified by in vivo imaging system, immunohistochemistry or other methods.

Relying on our expertise in extensive analysis, identification and combination technologies, in the validation of αβ TCR-γδ T cells, Creative Biolabs can verify candidate molecules by combining a variety of experimental methods. If you are interested in our services, please feel free to contact us for more detailed information.


  1. Eriksson, E. M., Davey, M. S. γδ T cells take the stage. Clinical And Translational Immunology. 2019, 8(11).
All listed services and products are for research use only. Do not use in any diagnostic or therapeutic applications.

Online Inquiry