γδ T Cell Phenotypic Characterization

γδ T cells are innate immune cells expressing a T cell receptor (TCR). It can express Vδ1 chains which are prominent in the intraepithelial layer of mucosal surfaces, where they are involved in maintaining epithelial tissue integrity when facing damage, infection, or transformation, responding to stress antigens on epithelial cells, and producing interleukin-10 (IL-10). Creative Biolabs provides high-quality services about γδ T cells, such as T cell memory and target identification for vaccine design, in vivo activation and tumor-targeting, the optimization of in vitro expansion protocols, and the development of gene-modified γδ T cells.

Phenotypic Characterization of γδ T-Cell Subsets

Cytotoxicity of human Vγ9Vδ2 T cells is dependent on the NKG2D activation and is mediated by Fas/Fas ligand (FasL) and perforin-granzyme B pathways. Compared the levels of surface NKG2D and FasL expressions with the intracellular expression of cytotoxic granules for the CD56+ and CD56-Vγ9Vδ2 T-cell subsets, we found that CD56+ and CD56- γδ T-cell subsets expressed with similar levels of NKG2D, perforin, granzyme B, and FasL, as well as CD69, which is a recent activation marker. CD56+ Vγ9Vδ2 T cells expressed a significantly higher level of CD16 than CD56- counterparts.

Fig.1 Phenotypic characterization of CD56+ and CD56- γδ T-cell subsets. (G, 2012)

Our Services

As a leader in the research of γδ T cells, Creative Biolabs is driven to provide comprehensive γδ T cells development and engineering services to help regulate immune responses. To better identify different subsets of γδ T cells, we also provide γδ T cells phenotypic characterization services.

• Monoclonal Antibody Service

Monoclonal antibodies play an important role in science experiments. According to your needs, we help you to prepare high purity antigen, formulate reasonable immune program, and provide you with specific monoclonal antibody by hybridoma technology. If you need us to prepare a large number of monoclonal antibodies about γδ T cell phenotypic characterization, we have in vivo induction method and in vitro culture method.

• Flow Cytometry (FCM)

FCM is a rapid and multi-parameter quantitative analysis of a single cell or other biological particles at the cellular and molecular level. According to your needs, we can provide relative quantitative or absolute quantitative analysis, polychromatic fluorescence analysis; cell membrane analysis and intracellular components and molecular phenotype analysis about different subsets of γδ T cells. We can also combine FCM with immunophenotyping to provide a very useful tool for the detection of specific nucleic acid sequences (such as oncogenes, viral nucleic acids, etc.) of selected cell subsets.

• Immunophenotype Analysis

By using specific antibodies, individual T cell subtypes can be distinguished in a heterogeneous immune cell pool. This analysis of immunocyte subtypes is called immunophenotypic analysis.

The basis of T cell immunophenotyping is that T cells have specific cell surface receptors, intracellular cytokine expression profiles and / or other intracellular proteins with different phosphorylation. This allows these cells to be selectively labeled with specific markers. According to the expression of CD3, the immunophenotype of T cells can be analyzed by immunohistochemistry (IHC) method, and then further subdivided according to other specific markers.

Why Us

• We offer a comprehensive range of services characterized by quality, predictability, delivery, and customer service.
• At project kick-off, we guarantee a turnaround time for each step of the process. In case of questions, our staff guarantees to reply within 24 hours.
• Our preclinical research programs are conducted in our state-of-the-art facility by a team of experienced and well-trained technicians to help maximize the value of your research investment.

With access to advanced instruments, fit-for-purpose laboratories, and professional knowledge, Creative Biolabs provides customized solutions to meet the needs of our clients. We deliver high-quality data by applying specific criteria during the process to ensure robustness and reproducibility. If you are interested in γδ T cell phenotypic characterization services, please contact us or directly sent us an inquiry.

Reference

1. Qin, G.; et al. Phenotypic and functional characterization of human γδ T cell subsets in response to influenza A viruses. International Journal of Infectious Diseases. 2012.