γδ T Cell Functional Characterization

γδ T cells are involved in immune surveillance against hematological neoplasms and solid tumors and the pathogenesis of autoimmune diseases. Vδ2 T cell responds mainly to intracellular bacteria and solid tumors, while Vδ1 T cell, resident mainly in tissues, is used mostly in the defense against viruses and malignancies. γδ T cells are being explored in cell-based immunotherapy. Creative Biolabs provides customers with isolation and test services of γδ T cells. Enriched γδ T cells can be prepared by magnet separation and the purity of γδ T cells is up to 85% to 90%, as assessed by flow cytometry analysis, and their cell viability is up to 90% as determined by trypan blue exclusion.

Function of Zebrafish γδ T Cells in Vitro

An in vitro antigen-specific CD4+ T cell activation assay was performed to investigate whether zebrafish γδ T cells can act as antigen-presenting cells (APCs) to initiate adaptive immunity. The distribution of major histocompatibility complex (MHC-II), CD80/86, and CD83, three specific APC markers involved in Ag presenting and costimulatory signaling to CD4+T cells, were determined in the activated γδ T cells magnetically sorted from the antigen (KLH)-stimulated fish through reverse transcription PCR (RT-PCR) and double-immunofluorescence staining. This result provides initial evidence that zebrafish γδ T cells exhibit the functional characteristics of APCs.

Fig.1 In vitro evaluation of the Ag-presenting capacity of zebrafish γδ T cells. (Feng, 2016)

Our Services

Creative Biolabs has rich experience in the research of γδ T cells. We can provide comprehensive γδ T cells services. In order to better understand the potential function of γδ T cells, we also provide γδ T cells functional characterization service as follows.

• Cell Culture Services

After the cells of interest (such as γδ T cells) have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. According to your experiments' needs, we can provide the specific cells or give you the most credible guidance for your cultured cells.

• Flow Cytotechnique (FCM)

FCM not only measures the size of cells, the character of internal particles, but also the surface and plasma antigen of cells. It is widely used in hematology, immunology, oncology, pharmacology, molecular biology and other disciplines. We provide a full range of FCM testing services, such as the testing of the relationship between cell cycle or DNA ploidy and cell surface receptor and antigen expression, classification and purification of immunocompetent cells.

• Cytokine Detection

γδ T cells can produce different cytokines. We provide you with three methods (immunoassay methods, bioassay, molecular biological methods) to detect cytokines. You can choose one according to the actual needs, or you can tell us your general test plan, and we will provide you with the combination test.

Why Us

• Structured Efficiency: Creative Biolabs' manufacturing facility is reflecting strong product quality and manufacturing procedures and ongoing improvement in those areas.
• Proven Experience: Over 10 years of experience, gives us the know-how and expertise that is needed to meet and exceed your expectations.
• Trustworthy Convenience: A fully automated Laboratory Management System enables us to work faster and more efficiently. Moreover, it gives you a user-friendly tool to order easily, with full insight into your order status.

Creative Biolabs has built a team of experienced scientists with specially designed facilities. Our comprehensive services include but are not limited to γδ T cell functional characterization. If you are interested in γδ T cells services, please contact us or directly sent us an inquiry.

Reference

1. Feng, W.; et al. Characterization of γδ T Cells from Zebrafish Provides Insights into Their Important Role in Adaptive Humoral Immunity. Frontiers in Immunology. 2016, 7.